EFFECT OF SOLVENT CONCENTRATIONS ON QUANTITATIVE ESTIMATION OF TOTAL PHENOLIC AND FLAVONOID CONTENTS OF AERIAL PARTS OF CALOTROPIS PROCERA (Ait.) R.Br

Document Type : Original research articles

Authors

1 Department of Pharmacognosy, Faculty of Pharmacy, Assuit University, Assuit , Egypt

2 Centre for Pharmaceutical Studies and Research of Medicinal Plants, Faculty of Pharmacy, Assuit University, Assuit , Egypt

3 Centre for Pharmaceutical Studies and Research of Medicinal Plants, Faculty of Pharmacy, Assuit University, Assuit, Egypt

4 (Head of department of Pharmacognosy, Faculty of Pharmacy,Sphinx University, New Assiut), (Faculty of Pharmacy, Assiut University, Assiut)

Abstract

Phenols and flavonoids present in medicinal plants are considered to be among the most important bioactive components. Calotropis procera (Ait.) R.Br. (Family, Apocynaceae) is a medicinal plant which showed a wide range of bioactivity. The aim of this study was to evaluate the effect of solvent concentration on total phenolic content (TPC) and total flavonoid content (TFC) in different extracts of leaves, stems and fruits of C. procera. TPC and TFC were estimated by Follin Ciocalteu colorimetric assay and aluminum chloride method using tannic acid and quercetin as standards, respectively. This study showed significant variation in TFC and TPC levels in between the different samples. C. procera presented highest both TPC and TFC in leaves extracted with 80% aqueous MeOH. In conclusion, the present investigation demonstrates that there is significant effect of solvent concentration used for extraction of aerial parts of C. procera on their TPC and TFC contents.

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